Gallery

Transmission electron micrographs of MDCK cells stably expressing Par3-APEX2-EGFP. APEX2 labeling reveals that Par3 localises specifically to tight junctions. Left: Longitudinal (en face) section through the epithelial monolayer. Right: Cross-section through the epithelial monolayer.

Confluent and fully polarised MDCK cells stained with antibodies against the polarity proteins Par3 and Pals1

MDCK cell cysts stably expressing a Pals1-APEX2-EGFP fusion protein grown in a 3D matrix of Collagen-I. Cysts were counterstained with the actin dye phalloidin.

MDCK cell cysts grown in a 3D matrix of Collagen-I. Cysts were fixed, stained with antibodies against E-Cadherin and the actin dye phalloidin, and imaged by spinning disk microscopy.

Transmission electron micrograph of caveolar rossettes in RPE cells. The sample was fixed in the presence of ruthenium red, which reveals surface-connected caveolae

Transmission electron micrograph of caveolae at the ventral membrane of mouse embryonic fibroblasts. Cells were transfected with a caveolin-1-APEX2-EGFP fusion protein to selectively visualise caveolae

Tomographic reconstruction of caveolar networks in RPE cells stably expressing the caveolar protein Cavin3 fused to a miniSOG probe. MiniSOG permits the specific staining of caveolae for EM.

The caveolar protein cavin-1 forms oligomeric assemblies. HeLa cells were crosslinked live with the indicated concentrations of the cell-permeable crosslinker DSP. Cell lysates were separated by SDS-PAGE in the absence of reducing agent to maintain the crosslinks

Transmission electron micrograph of HeLa cells infected with RSV and transfected with caveolin-1-APEX2-EGFP. Caveolae are concentrated at the base of RSV filaments

Transmission electron micrograph of a centriole in MDCK cells

Transmission electron micrograph of a basal body in MDCK cells

Transmission electron micrograph of a Golgi apparatus in MDCK cells.

Transmission electron micrograph of sinusoidal ER in HeLa cells

Transmission electron micrograph of a clathrin coated pit in MDCK cells.

Transmission electron micrographs of different stages of clathrin coated pit formation and budding in MDCK cells.

Dancing RPE cells. Cells were stably transfected with a cavin-3-mCherry fusion protein and imaged live with a spinning disk microscope

RPE cells stably transfected with a cavin-3-mCherry fusion protein imaged live with a spinning disk microscope

RPE cells stably transfected with cavin-3-mCherry embedded in a collagen-I matrix and imaged live with a spinning disk microscope