Gallery

Confluent and fully polarised MDCK cells stained with antibodies against the polarity proteins Par3 and Pals1

Stages of apical lumen initiation in MDCK-II cells embedded in Matrigel imaged by Airy Scan microscopy

 

MDCK cell cysts stably expressing a Pals1-APEX2-EGFP fusion protein grown in a 3D matrix of Collagen

 

Transmission electron micrographs of MDCK cells stably expressing Par3-APEX2-EGFP. Left: Longitudinal (en face) section through the epithelial monolayer. Right: Cross-section through the epithelial monolayer.

 

Par3, tight junctions, APEX2

 

Transmission electron micrograph of MDCK cells stably transfected with Par3-APEX2-EGFP. Note the distinct membrane contrast produced by APEX2 labeling.

Transmission electron micrograph of caveolae at the ventral membrane of mouse embryonic fibroblasts visualised with a caveolin-1-APEX2-EGFP fusion protein 

Transmission electron micrograph of RSV-infected HeLa cells transfected with caveolin-1-APEX2-EGFP. Caveolae are concentrated at the base of RSV filaments

Transmission electron micrograph of caveolar rossettes in RPE cells

 

Transmission electron micrograph of sinusoidal ER in HeLa cells

 

Transmission electron micrograph of a centriole in MDCK cells

 

Transmission electron micrograph of a basal body in MDCK cells

 

Transmission electron micrograph of a Golgi apparatus in MDCK cells

 

Transmission electron micrograph of a clathrin coated pit in MDCK cells

 

Transmission electron micrographs showing different stages of clathrin coated pit formation and budding

 

Transmission electron micrographs of tight junctions in wild-type and Arhgap12 KO MDCK cells

RPE cells stably transfected with a cavin-3-mCherry fusion protein imaged live with a spinning disk microscope

Note that caveolae (labeled by cavin3) are concentrated at and stably associated with the cell rear.

Tomographic reconstruction of caveolar networks in RPE cells stably expressing the caveolar protein Cavin3 fused to a miniSOG probe

Caveolae form large interconnected networks at the rear of migrating cells, providing a membrane reservoir during cell rear retraction.

Cryo Electron Tomography of the perinuclear region in RPE1 cells

The nuclear envelope, a nuclear pore, the Golgi apparatus, ER tubules, ribosomes, and vesicular structures are visible.